Anti-PSMD9; clone 16C5.1

Application

Research CategoryNeuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PSMD9 in 10 µg of HepG2 cell lysate.
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected PSMD9 in normal human kidney and prostate tissues, as well as human prostate cancer tissue sections.

Anti-PSMD9 Antibody, clone 16C5.1 is an antibody against PSMD9 for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

26S proteasome non-ATPase regulatory subunit 9 (UniProt O00233; also known as 26S proteasome regulatory subunit p27, Homolog of rat Bridge 1, Proteasome macropain non-ATPase subunit 9) is encoded by the PSMD9 gene (Gene ID 5715) in human. PSMD9 is a proteasomal assembly chaperone that forms a stable subcomplex with two AAA-ATPases, PSMC3 and PSMC6, in assisting the assembly of the 20S and 19S particles to form the holo complex. PSMD9 contains a PDZ-like domain (a.a. 108-195) that is reported to interact with a short 4- to 7-amino acid linear sequence motifs (SLIMs) at the C-terminal end of its client proteins, including the RNA-binding protein hnRNPA1, the ribosomal protein S14, the growth hormone CSH1, the transcription factor E12, and the IL6 receptor. PSMD9 and hnRNPA1 interaction is found to play a role in IκBα proteasomal degradation, where hnRNPA1 functions as shuttle receptor that recruits IκBα to the proteasome for degradation.

Immunogen

GST-tagged recombinant human PSMD9 internal fragment.

Epitope: Internal.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSMD9 in 10 µg of SHSY-5Y cell lysate.

Specificity

Expected to react with spliced isoforms p27-L and p27-S, but not spliced isoform 3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~26 kDa observed. 24.68 kDa (isoforms p27-L) and 22.76 kDa (isoforms p27-S) calculated.