Application

Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-7).

Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-7; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).

Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate of P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).

Anti-Dab1, clone 4H11, Cat. No. MABN2277, is a highly specific rat monoclonal antibody that targets Disabled homolog 1 and has been tested for use in Immunoprecipitation, Immunohistochemistry, and Western Blotting.

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Disabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species. Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer. It is a cytosolic protein that is shown to be essential component of the reelin signaling pathway. It is shown to bind to the cytoplasmic tail of Reelin receptor, APO E receptor 2, and to LDL receptor. Dab1 can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons. It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling. Tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling. Dab1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins. Seven different isoforms of Dab1 have been identified that are generated via alternative splicing. Clone 4H1 binds to an epitope within amino acids 281-460. (Ref.: Onoue, A., et al. (2014). Neurosci. Res. 88; 23-27).

Immunogen

GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293T cells expressing murine Dab 1.


Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Dab1 in lysates from HEK293T cells expressing murine Dab 1 but not in mock HEK293T cells.

Specificity

Clone 4H11 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

68 kDa observed; 63.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).