Analysis Note

ControlRat brain lysate

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Detect HCN1 using this Anti-HCN1 Antibody, clone N70/28 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected HCN1 in rat cerebellum and rat brain/inferior colliculus tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 proteins play an important role for motor learning and synapse formation through integration of cerebellar Purkinje cells. The two proteins also shape autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is particularly high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization of activated cation channels of the HCN gene family such as HCN1, contributes to spontaneous rhythmic activity in both heart and brain. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.

Immunogen

Epitope: Cytoplasmic domain

Recombinant protein corresponding to rat HCN1 at the cytoplasmic domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Western Blot in rat brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected HCN1 on 10 µg of rat brain lysate.

Specificity

This antibody recognizes HCN1 at the cytoplasmic domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed; consistent with a glycosylation of a 100kDa protein.