ANTI-AMYLOID BETA ANTIBODY; CLONE W0-2

Analysis Note

ControlHuman Alzheimer diseased brain tissue lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Amyloid β in Alzheimer's diseased hippocampus.

Detect Amyloid β using this Anti-Amyloid β Antibody, clone W0-2 validated for use in WB, IH, ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The cerebral and vascular plaques associated with Alzheimer′s disease (AD) are mainly composed of amyloid beta peptides (Aβ). Aβ is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Aβ [1-40], Aβ [1-42], and Aβ [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last APP processing step. Aβ [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. These Amyloid beta antibodies and peptides have been developed as tools for elucidating the biology of AD.

Immunogen

Human Amyloid β peptide

Epitope: Amino acid residues 4-10

Linkage

Replaces: MAB1561

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in human Alzheimer disease brain tissue lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Amyloid β in 10 µg of human Alzheimer diseased brain tissue lysate.

Specificity

This antibody recognizes amino acid residues 4-10 of human Amyloid β.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Calculated MW is 4 kDa This antibody can also show 120 and 105 kDa APP proteins, and ~45 kDa oligomers of amyloid proteins on our western blot of human Alzheimer diseased brain lysate.