Application

Anti-CD6, clone 34-3, Cat. No. MABF2091, is a rat monoclonal antibody that detecs T-cell differentiation antigen CD6 and has been tested for use in Flow Cytometry.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

T-cell differentiation antigen CD6 (UniProt: Q61003; also known as CD6) is encoded by the Cd6 gene (Gene ID: 12511) in murine species. CD6 is a single-pass type I membrane protein that is detected at the immunological synapse the contact zone between antigen-presenting dendritic cells and T-cells. It functions as a costimulatory molecule and promotes T-cell activation and proliferation. After T-cell activation, CD6 is hyperphosphorylated on serine and threonine residues and it is also phosphorylated on tyrosine residues in response to stimulation of the TCR complex. CD6 is predominantly expressed in thymus, lymph node, and spleen. It mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It also contributes to signaling cascades triggered by activation of the TCR/CD3 complex. It functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. It binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. It mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 is synthesized with a signal peptide (aa 1-16), which is cleaved off to produce a mature form, which has an extracellular domain (aa 17-398), a transmembrane region (aa 399-419), and a long cytoplasmic tail (aa 420-665).

Immunogen

Epitope: extracellular domain

CD6-transfected NB2 cells

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD6 in one million mouse splenocytes.

Specificity

Clone 34-3 is a rat monoclonal antibody that detects T-cell differentiation antigen CD6 in murine species.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

72.26 kDa calculated.