Analysis Note

ControlIMR-32 cell lysate

Application

Research Sub CategoryTranscription Factors

Use Anti-N-Myc Antibody, clone NCM II 100 (mouse monoclonal antibody) validated in WB, IP, ICC to detect N-Myc also known as N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected N-Myc in IMR5 cells (Ikegaki, N., et al. (1986). 83(16):5929-5933.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated N-Myc in IP (Brondyk, W. H., et al. (1991). 6(7):1269-1276.).

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.

Immunogen

Recombinant protein corresponding to human N-Myc.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected N-Myc in 10 µg of IMR-32 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~55 kDa observed. Uniprot describes a molecular weight at ~50 kDa Uniprot states that this protein may be phosphorylated.