Anti-KDM5B; clone 15G8.1

Application

This Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.

General description

Lysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.

Immunogen

GST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.

Epitope: Region between ARID and JmjC domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.

Target description

~175 kDa observed. Uncharacterized band(s) may appear in some lysates.