Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting Analyis: A representative lot from an independent laboratory detected XBP-1S, clone 143F (active form) in SK-MM-2, KARPAS 620, NCI-H929, and LP-1 cell lysates (Maestre, L., et al. (2009). Haematologica. 94(3):419-422.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected XBP-1S, clone 143F (active form) in human tonsil and lymphoplasmacytic cells (Maestre, L., et al. (2009). Haematologica. 94(3):419-422.).

Anti-XBP1s Antibody, clone 143F (active form) is an antibody against XBP1s for use in IHC & western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

XBP-1, also known as X-box-binding protein 1 or Tax-responsive element-binding protein 5, and encoded by the gene XBP1/TREB5/XBP2, is a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP-1 also acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). XBP-1 binds DNA preferentially at CRE-like elements but also to some TPA response elements (TRE). XBP-1 is expressed in the nucleus. Defects in XBP-1 may be associated with Major affective disorder 7 (MAFD7) a major psychiatric disorder that is characterized by severe mood swings, with fluctuation between two abnormal mood states (manic or major depressive episode). Mania is accompanied by symptoms of euphoria, irritability, or excitation, whereas depression is associated with low mood and decreased motivation and energy.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human XBP-1S, clone 143F (active form).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunohistochemistry in human skin tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected XBP-1S, clone 143F (active form) in human skin tissues.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

29 kDa calculated