Analysis Note

ControlBrain tissue

Application

Western blotting on recombinant alpha synuclein: 1:2,000-1:4,000.

Lane 1: Recombinant human alpha synuclein

Lane 2: Recombinant human beta synuclein

Lane 3: Recombinant human gamma synuclein

Immunohistochemistry (frozen sections): 1:250-1:500

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Anti-Synuclein Antibody, α, clone 7B12.2 detects level of Synuclein & has been published & validated for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant human alpha synuclein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1053

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

UnPurified tissue culture supernatant from a perfusion system, filtered through a 0.2µ micron membrane prior to vialing. Product contains 20ûS and Ciprofloxacin at final concentration of 10µg/mL.

Unpurified

Specificity

Reacts with alpha Synuclein. By Western blot the antibody shows no reactivity with beta or gamma synuclein. This antibody was shown to stain Lewy bodies in frozen section of human brain with Parkinson′s disease and glial inclusions in frozen section of human brain with Multiple System Atrophy.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

15-18 kDa