Application

Research CategoryApoptosis & CancerMetabolism

Detect Caspase 8 using this Anti-Caspase 8 Antibody, clone 1-1-37 validated for use in WB.

Western blot: 1:100 - 1:1000

Optimal working dilutions must be determined by end user.

Research Sub CategoryCaspasesEnzymes & Biochemistry

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Caspase-8 is a 55 kDa protein that binds to the death effector domain (DED) of FADD through an analogous DED domain present in the proform of caspase-8. Recruitment of caspase-8 to the Fas receptor results in oligomerization of the caspase-8 protein, which drives its auto-activation through self-cleavage. Active caspase-8 then proteolytically activates other downstream caspases including caspase-9, which lead to cell death.

Immunogen

Recombinant full-length human caspase-8 protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-573; 04-574

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.

Format: Purified

Specificity

Highly specific to caspase-8 and shows no cross-reaction with other caspase family members. This antibody recognizes the full length 55kDa caspase 8 on western blots.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.