NGF RECPT; MS X RT-50UG

Analysis Note

ControlWestern Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.

Application

Western Blot:
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.

Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.

Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.

Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.

Research CategoryNeuroscience

Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.

Research Sub CategoryNeurochemistry & Neurotrophins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.

Immunogen

n-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984).

Epitope: Extracellular

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1111

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Mouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide

Format: Purified

Quality

Immunohistochemistry:
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.

Specificity

Specific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75.

Reacts with rat p75 protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

75 kDa