Analysis Note
ControlPositive Control: U251 or D54 human cell lines. Membrane preps are recommended for western blots.
Human endothelium cells (expression of integrin alpha V beta 3 may need to be upregulated by phorbol esters stimulation). Skin (Basement Membrane)
Application
Research Sub CategoryIntegrins
Flow cytometry
Function-blocking of integrin beta3-mediated adhesion to ECM proteins.
Western blot (reducing conditions) Optimal working dilutions must be determined by end user.
Research CategoryCell Structure
Anti-Integrin β3 Antibody, clone B3A, azide free detects level of Integrin β3 & has been published & validated for use in FC, FUNC & WB.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human integrin alphaVbeta3 heterodimer isolated from placenta.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Format: Purified
Specificity
Specific for human integrin beta 3 (other species not tested).
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Target description
110 kDa
This product has met the following criteria to qualify for the following awards: