Anti-Fmc-7 (B-Cell Lymphocyte Marker);

Application

Flow Cytometry Analysis: 4.0 µg of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in peripheral blood mononuclear cells.

This Anti-Fmc-7 (B-Cell Lymphocyte Marker) antibody is validated for use in WB, FC for the detection of Fmc-7 (B-Cell Lymphocyte Marker).

General description

B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.

Immunogen

HRIK [human B-lymphoblastoid cell] corresponding to human Fmc-7 (B-Cell Lymphocyte Marker).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgMκ in buffer containing 0.1M Tris, 10mM Glycine, 0.5M NaCl, and 0.1% Sodium Azide.

Format: Purified

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in 10 µg of THP-1 cell lysate.

Target description

~105 kDa observed. Uncharacterized band(s) may appear in some lysates.