BONE SIALOPROT; MS X HU-100UG

Application

Research Sub CategoryOsteobiology

Western blot: (1µg/mL)

Immunohistochemistry on paraffin embedded tissue sections: (1µg/mL)

Immunocytochemistry: (1:100)

ELISA: (1µg/mL)

RIA

Optimal working dilutions must be determined by the end user.

Research CategoryCell Structure

This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.

Immunogen

Bone Sialoprotein isolated from human bones.

Epitope: C-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1855

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.

Format: Purified

Specificity

Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.

Storage and Stability

Maintain lyophilized material at 2-8°C. Reconstitute with 100µL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.