Application
Research Sub CategoryCytokines & Cytokine Receptors
Capture antibody in ELISA. Recommended coating concentration 5 µg/mL. See suggested protocol.
Flow cytometry: use 1 µg/test
Neutralizes recombinant ovine IL-8.
Western blotting
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-8
1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 µg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4°C.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 µL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 µL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 µL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 µL of 2M sulfuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Research CategoryInflammation & Immunology
This Anti-Interleukin-8 Antibody, clone 8M6 is validated for use in ELISA, FC, NEUT, WB for the detection of Interleukin-8.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Recombinant ovine IL-8
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid in PBS containing 0.1% sodium azide.
Format: Purified
Specificity
Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP-1 or TNFalpha.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
This product has met the following criteria to qualify for the following awards: