ANTI-SIPA1

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Signal-induced proliferation-associated protein1 (SIPA1) helps in the hydrolysis of Rap1 bound GTP (guanosine triphosphate) to GDP (guanosine diphosphate). It provides GAP activity for Ras-related regulatory proteins Rap1 and Rap2. SIPA1 controls cell progression, differentiation, adhesion and cancer metastasis. It serves as a signal transducer to activate the Ras-related proteins. In human oral squamous cell carcinoma, SIPA1 induces invasion and migration with the help of ITGB1 (integrin beta1) and MMP7 (matrix metalloproteinase 7).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

General description

Signal-induced proliferation-associated protein 1 (SIPA1) is a GTPase activating protein. This gene is located on human chromosome 11q13.1. The gene spans around 12.8kb and has 16 exons. SIPA1 has 1042 amino-acids and is of 130kDa. It has a Rap GTPase-activating (GAP) domain (350-539), PDZ domain (685-759) and leucine zipper like domain (964-1042). SPA-1 is widely expressed in lymphohematopoietic tissues like bone marrow, thymus and spleen.

Immunogen

signal-induced proliferation-associated 1

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST80847.

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.