Anti-EGFR (Ab-1) Mouse mAb (528)

Analysis Note

Positive ControlA-431 cells or skin tissue

Application

Flow Cytometry (5 µg/ml) r>Frozen Sections (2.5 µg/ml) r>Immunofluorescence (5 µg/ml) r>Immunoprecipitation (1 µg/sample)r>Neutralization Studies (see comments and application references)r>Paraffin Sections (see application references)

General description

Recognizes the EGF receptor protein in A431 cells and normal skin.

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.

Immunogen

partially purified, human EGFR from human A431 cells

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hunter T. 1984. Nature311, 414.Masui, H., et al. 1984. Cancer Res.44, 1002.Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Warning

Toxicity: Standard Handling (A)