Analysis Note

ControlHuman Lymphocytes

Application

Research Sub CategoryImmunoglobulins & Immunology

Detect CD71 using this Milli-Mark Anti-CD71-FITC Antibody, clone Ber-T9 validated for use in FC.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD71 is a 190 kDa disulfide-bonded homodimeric type II transmembrane glycoprotein. CD71 is the receptor for transferrin. CD71 is involved in iron (Fe3+) uptake, and expression is regulated by the metabolic demand for iron, which is essential for the growing cell (Goding, 2002). CD71 is more abundantly expressed in rapidly dividing cells than quiescent cells (Newman, 1982; Sutherland, 1981). In normal tissues, high expression of CD71 is seen in the placental trophoblast and hemoglobin-synthesizing reticulocytes (Newman, 1982) but expression is lost in mature erythrocytes (Sutherland, 1981). Anti-CD71, Ber-79, was included in the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD71 (Boeker, 1995).

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human lymphocytes

Specificity

Antibody recognizes Human CD71 antigen.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

190 kDa (Goding, 2002)