Analysis Note

Negative Control293T cells

Positive ControlA431 cells, Human leiomyosarcoma tissue, HeLa cells

Application

Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (5 µg/ml)

General description

This Anti-TARDBP Mouse mAb (2E2-D3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TARDBP.

Recognizes the ~45 kDa TARDBP protein in A431 and HeLa cells and human leiomyosarcoma tissue.

Purified mouse monoclonal antibody. Recognizes the ~45 kDa TARDBP protein.

Immunogen

Full-length, recombinant human TARDBP (aa 1-261), expressed as a GST fusion protein.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ilieva, E,V., et al. 2010. Free Radic. Biol. Med.48, 1302.Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A107 3858.Foulds, P,G., et al. 2009. Acta Neuropathol.118 647.

Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.7.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)