Analysis Note

Positive ControlHeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

Recognizes the SQSTM1 protein HeLa cells.

Purified mouse monoclonal antibody. Recognizes the SQSTM1 protein.

This Anti-SQSTM1 Mouse mAb (2C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of SQSTM1.

Immunogen

NA

Full-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lee, H,K., et al. 2010. Immunity.32, 227.Bjorkoy, G., et al. 2009. Methods Enzymol.452, 181.Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet.18 4868.Gal, J., et al. 2009. J. Neurochem.111 1062.Mardakheh, F,K., et al. 2009.J. Cell Biol.187, 265.Jung, H,S., et al. 2008. Cell Metab.8, 318.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)