Analysis Note

Positive ControlHeLa cells, Human endometrium tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (3 g/ml)

General description

Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.

This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.

Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.

Immunogen

NA

A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Mistry H, et al. 2008. Biochem. Biophys. Res. Commun.375 321.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)