Application

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.

Detect Rat IgG using this Goat anti-Rat IgG Antibody, HRP conjugate validated for use in ELISA, IH & WB.

General description

Goat anti-rat IgG affinity purified antibody, HRP conjugated.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitute with 2 mL of sterile distilled water.

Quality

Routinely evaluated on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..

Specificity

Specific for rat IgG, heavy and light chain.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.