Application

Research Sub CategoryCytoskeleton

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected CAPN6 in human lung tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected CAPN6 in human lung cells.

Research CategoryCell Structure

Anti-CAPN6 Antibody is an antibody against CAPN6 for use in western blotting, IHC & immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calpain-6, also known as Calpain-like protease X-linked or Calpamodulin (CalpM) and encoded by the gene name CAPN6 or CALPM, belongs to a well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. CAPN6 is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.

Immunogen

KLH-conjugated synthetic peptide corresponding to the C-terminus of human CAPN6.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat lung tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CAPN6 in rat lung tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed. Uncharacterized bands may be observed in some lysate(s).