Analysis Note

ControlRabbit quadraceps tissue

Application

Detect Sarcolipin using this Anti-Sarcolipin Antibody validated for use in Western Blotting.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sarcolipin (SLN) is a multidomain protein containing lumenal, cytoplasmic, and transmembrane domains. It is found in the membrane of the sarcoplasmic/endoplasmic reticulum of cardiac cells, and is involved in maintaining calcium homestasis underlying muscle contractility in the heart. Sarcoplipin achieves these effects through its regulation of the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2a), a calcium transport protein that actively pumps calcium from the cytoplasm into the sarcoplasmic reticulum, resulting in relaxation of the heart muscles. Sarcolipin may also interact with phospholamban, and may contribute to heart failure.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in rabbit quadraceps tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Sacolipin in rabbit quadraceps tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~6 kDa observed. An uncharacterized band may be observed at ~13 kDa in some tissue lysates.