Application

This Anti-Krueppel-Like Factor 4 (Klf4) antibody is validated for use in WB, IC for the detection of Krueppel-Like Factor 4 (Klf4).

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Krueppel-Like Factor 4 (Klf4) in HeLa, A431, and NIH/3T3 cells.

General description

Krueppel-like factor 4 (KLF4), which is also known as epithelial zinc finger protein (EZF) and gut-enriched krueppel-like factor (GKLF), is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and is used as a marker of the pluripotent state for cells in culture. It is required for the differentiation of epithelial cells and the establishment of the barrier function of the skin, and KLF4 is also expressed during skeletal and kidney development. KLF4 is highly expressed in the skin, lung, and intestine along the epithelial border. This nuclear localized protein can act as both a tumor suppressor and activator depending upon its environment, but its most prominent function is growth suppression. Interestingly, increased expression of KLF4 leads to an increase in expression of P21WAF1/CIP1 which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries. Additionally, KLF4 mediates the normal functions of the tumor suppressor p53 as well. Mutations thus in KLF4 lead to unchecked growth and tumor formation, and because of its expression and involvement within the gut, KLF4 is often mutated in many colon cancer cell lines and the lack of KLF4 expression is correlated with colorectal cancer pathogenesis. Elevated expression of KLF4 gene expression has also been associated with gastric cancer, breast cancer, and squamous cell carcinomas. EMD-Millipore's Anti-KLF4 mouse monoclonal antibody, clone 1E6, has been tested in western blot against recombinant hKLF4 expressed cell lysates and in immunocytochemistry on paraffin embedded human lung and colon cancer tissue. The clone has also been tested in fluorescent immunocytochemistry on the human esophageal squamous carcinoma cell line Eca109 in culture.

Immunogen

GST & His-tagged recombinant protein corresponding to the N-terminus of human Krueppel-Like Factor 4 (Klf4).

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in mouse embryo stem cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Krueppel-Like Factor 4 (Klf4) in 10 µg of mouse embryo stem cell lysate.

Target description

~55 kDa observed