Anti-ELFN1

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ELFN1 in human thalamus and rat cerebral cortex tissue.

This Anti-ELFN1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ELFN1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ELFN1 is a postsynaptic protein that regulates circuit dynamics in the CNS by modulating the temporal dynamics of interneuron recruitment. ELFN1 is specifically expressed in excitatory synapses onto orens-lacunosum (OLM) interneurons and acts as a regulator of presynaptic release in directing of the pyramidal-OLM synapses. ELFN1 localizes to excitatory synapses on somatostatin containing OLM interneurons at the plasma membrane, and ELFN1 protein, expressed post-synaptically, can direct the elaboration of specific electrochemical properties of synapses between particular cell types in the hippocampus. ELFN1's expression is found in distinct subsets of interneurons of the hippocampus and cortex, and also in discrete subsets of cells in the habenula, septum, globus pallidus, dorsal subiculum, amygdala and several other brain regions in the mouse. The protein is also expressed in diverse cell types, including local GABAergic interneurons as well as long-range projecting GABAergic and glutamatergic neurons.

Immunogen

Epitope: Near N-terminal, extracellular domain

KLH-conjugated linear peptide corresponding to human ELFN1 near the N-terminal, extracellular domain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ELFN1 in 10 µg of U2OS cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~100 kDa observed