Anti-ZBTB38

Application

Anti-ZBTB38 Antibody, Cat. No. ABE1341, is a highly specific rabbit polyclonal antibody that targets ZBTB38 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ZBTB38 in human testis and human kidney tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger and BTB domain-containing protein 38 (UniProt: Q8NAP3, ZBTB38) is encoded by the ZBTB38 gene (Gene ID: 253461) in human. ZBTB38 is a transcriptional regulator with bimodal DNA-binding specificity. It binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5′-CGCG-3′, but can also bind to E-box elements (5′-CACGTG-3′). It can also bind specifically to a single methyl-CpG pair and represses transcription in a methyl-CpG-dependent manner. ZBTB38 protein is reported to play an important role in regulating DNA replication and common fragile sites (CFS) stability in a RBBP6- and MCM10-dependent manner and is shown to repress expression of MCM10, which plays an important role in DNA-replication. It is also believed to be involved in the differentiation and/or survival of late post-mitotic neurons. ZBTB38 protein contains ten C2H2-type zinc fingers and its BTB (Broad-Complex, Tramtrack and Bric a brac) domain is localized in the amino acids 33-100. However, the BTB domain is not required for its activation. ZBTB38 is down-regulated under conditions of endoplasmic reticulum (ER) stress, which promotes ER stress-associated apoptosis in human bone marrow neuroblastoma cells. In mice subjected to spinal cord injury (SCI) a repression of ZBTB38 is observed and this is linked to reduced secondary tissue damage after SCI. (Ref.:Cai, Y., et al. (2017). Oncotarget 8(28); 45356-45366).

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminus region of human Zinc finger and BTB domain-containing protein 38.

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ZBTB38 in HeLa cell nuclear extract.

Specificity

This rabbit polyclonal antibody specifically detects Zinc finger and BTB domain-containing protein 38 in human cells. It targets an epitope within 20 amino acids from the C-terminus region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~170 kDa observed; 134.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).