Application

Research CategoryApoptosis & Cancer

This Anti-IGF2 Antibody is validated for use in Western Blotting for the detection of IGF2.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human Insulin-like Growth Factor 2 (IGF2) contains 67 amino acids and shares similar structural features with IGF1, including a 62% sequence homology. In human plasma, IGF1 and IGF2 are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGF2 binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGF2 is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Immunogen

Epitope: Insulin-like growth factor 2 mature chain

KLH-conjugated linear peptide corresponding to the human Insulin-like growth factor 2 mature chain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IGF2 in 10 µg of human placenta tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~24 kDa observed. This antibody detects all 3 isoforms of IGF2 (20-26 kDa), and may run higher than expected due to glycosylation.