Anti-Bmf

Analysis Note

ControlHepG2 and HEK293 cell lysates

Application

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Bmf in HeLa cells.

Detect Bcl-2-modifying factor using this rabbit polyclonal antibody, Anti-Bmf Antibody validated for use in western blotting & IHC.

General description

Bcl-2-modifying factor (Bmf) is a member of the Bcl-2 family containing one BCL2 homology domain 3. Members of this family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell′s actin cytoskeleton. Bmf is constitutively expressed in many tissues. There are 3 isoforms of Bmf. Isoform 1 is mainly expressed in B-lymphoid cells while isoform 2 and 3 are mainly expressed in B-CLL and normal B-cells.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human Bmf.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting in HepG2 and HEK293 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Bmf in 15 µg of HepG2 and HEK293 cell lysates.

Specificity

This antibody recognizes the N-terminus of Bmf.

Target description

~25 kDa observed