Analysis Note

ControlHeLa, Jurkat, A431, and K562 whole cell lysates

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Detect Caspase 10 using this rabbit polyclonal antibody, Anti-Caspase-10 Antibody validated for use in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Caspase-10 is a member of the peptidase C14A family and is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is expressed in many tissues and cell lines with the lowest expression found in brain, kidney, prostate, testis, and colon. Mutations in Caspase-10 have been associated with autoimmune lymphoproliferative syndrome type 2A and gastric cancer.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Caspase-10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HeLa, Jurkat, A431, and K562 whole cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-10 in 15 µg of HeLa, Jurkat, A431, and K562 whole cell lysates.

Specificity

This antibody recognizes the C-terminus of Caspase-10.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~59 kDa observed. Uniprot describes 6 isoforms ranging from ~31 kDa to ~59 kDa Uncharacterized bands may appear in some lysates.