Application

Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.

Immunogen

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a registered trademark of Cytiva

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose^® 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

Specificity

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).