HCN4; RBX-200UL

Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 120 µg of control antigen. The stock solution of the antigen can be made up using 100 µL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of fusion protein with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

This Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry using rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

GST fusion protein corresponding to amino acids 119-155 of human HCN4 (Accession XP_007730, NP_005468, CAB42604, CAB52754).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 0.05% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4). The epitope does not share homology with any other known proteins.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.