SYNUCLEIN; ALPHA; RB X; AP-20UG

Analysis Note

ControlBrain tissue

Application

Research Sub CategoryNeurodegenerative Diseases

Western blot: 1:1000-1:2000

Immunohistochemistry: 1:1000-1:2000 on paraffin sections, 1:4000-1:12,000 on frozen sections..

Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

This Anti-Synuclein Antibody, α is validated for use in IH(P), WB for the detection of Synuclein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

alpha -Synuclein is a small (140-amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). alpha -Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including alpha-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

Immunogen

Human alpha-synuclein residues 111-131 coupled to KLH. The peptide sequence is N-GILEDMPVDPDNEAYEMPSEEC-C. This peptide is available for use in blocking studies (Cat. No. AG239).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1053

Physical form

Affinity Purified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 20 µL of sterile distilled water. Centrifuge to remove any residue.

ImmunoAffinity Purified

Specificity

Recognizes Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with beta-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.

Storage and Stability

Maintain at -20 in undiluted aliquots for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Target description

14-19 kDa