Application

Anti-IP3 Receptor Type II Antibody detects level of IP3 Receptor Type II & has been published & validated for use in IC, IF, IH, IP & WB.

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Western blot: 1:50 - 1:100 (detects a 260 kDa band)

Immunofluorescence: 1:10 - 1:20

Immunoprecipitation

Immunocytochemistry: Known to give positive reactivity on AR4-2J, HL-60, COS-I, HEK-293, and RBL-2H3 cell lines.

Immunohistochemistry: Paraformaldehyde or methanol fixes are effective.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified versus immobilized immunogen peptide. Liquid in PBS buffer, containing 0.05% BSA, 10% glycerol, 0.005% sodium azide. Caution: Sodium azide is hazardous. MSDS available upon request.

Specificity

Rabbit anti-Type II Inositol 1,4,5-Trisphosphate Receptor (IP3R) is directed against a synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor. The antibody is reactive with Type II IP3R from rat, mouse, human, hamster, and monkey.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.