Analysis Note

ControlMouse cerebellum lysate

Application

Research Sub CategoryGrowth Cones & Axon Guidance

Research CategoryNeuroscience

Anti-Nogo Receptor Antibody detects level of Nogo Receptor & has been published & validated for use in WB, IH.

Non-Lot Specific Tested Application 1:
Immunohistochemistry: Working dilutions of 1:50-1:500 in rat brain and spinal cord (paraffin embedded)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Axons are essential for neuronal communication but they do not regenerate after injury to the adult mammalian brain or spinal cord. Failed regeneration is due in part to the production of a potent axonal growth inhibitor, Nogo, by myelinating cells. The finding of a high affinity axonal receptor for the extracellular domain of Nogo provides the first insight into the basis of Nogo action. Disrupting the interaction of Nogo with the Nogo-66 receptor may facilitate axonal regeneration in vivo. The protein is dubbed the Nogo receptor because it binds with several other proteins that block neural growth. It is found to be ubiquitous in the brain and spinal cord.

Immunogen

Epitope: Mature Form

Recombinant protein.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in PBS with 0.1% NaN3

Protein A purified

Format: Purified

Quality

Routinely tested on mouse cerebellum lysate.
Lot Specific Tested Application 1: Western Blot

Specificity

May react with human and primate based on sequence homology. Reactivity with other species has not been determined.

Cat. # AB15138 recognizes the mature form of Nogo Receptor.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Target description

~50.9 kDa