Application

Anti-Tryptophan Antibody is an antibody against Tryptophan for use in IH.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Immunohistochemistry: 1:1,000-1:2,500 by PAP (see suggested protocol).

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Tryptophan-gluteraldehyde-Poly-lysine

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit antiserum. Liquid with 0.05% sodium azide.

Specificity

Tryptophan

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:

Compound Cross-reactivity
Tryptophan-G-PL 1
5-Hydroxytryptophan-G-PL .01
5-Methoxytryptophan-G-PL .004
Tryptophan=G=PL (non reduced) .002
Tryptophan .0003
Tryptamine-G-PL .00009
5-Methoxytryptamine-G-PL .00002
5-Hydroxytryptamine-G-PL .000004

The antisera was also tested for specificity using the free-floating PAP technique on rat brain.

Abbreviations:
(G)Glutaraldehyde
(=)Non-reduced conjugate
(PL)Poly-lysine

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.