Application

Immunoblotting: antibody recognizes alpha and beta subunits of MPO. Reduced and denatured extracts of purified MPO produce blots produce 55-60kDa alpha subunits and 12-14kDa beta subunit bands140 Additional bands may be present due to cross reactivity with other myeloperoxidases.

ELISA:

Optimal working dilutions must be determined by end user.

Anti-Myeloperoxidase Antibody, human white cell is an antibody against Myeloperoxidase for use in ELISA & WB.

Immunogen

Epitope: human white cell

Myeloperoxidase (Human White Cells)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Monospecific antiserum purified by delipidation and defibrination. Lyophilized from 0.02 M Potassium Phosphate buffer, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide. Reconstitute with 2.0 mL of sterile distilled water.

Specificity

Myeloperoxidase (Human White Cells). Monospecific by IEP. Some cross reactivity with Myeloperoxidase from other tissues and species may occur.

Myeloperoxidase (MPO) represents an early appearing and highly reliable intracellular myeloid lineage marker molecule. MPO protein can be detected in a subset of human hematopoietic bone marrow progenitor cells and in granulomonopoietic (GM) cells. However, other myeloid-related cell types such as epidermal Langerhans-type dendritic cells (LC) lack MPO. Therefore, human myeloid progenitors might be subdivided based on MPO protein expression into functional subsets.

Storage and Stability

Store unopened vial at 2-8 °C. Maintain frozen at -20°C in undiluted aliquots for up to one year after reconstitution. Avoid repeated freeze/thaw cycles.