General description

Native, PP2A₁ from bovine kidney. A divalent cation-independent protein serine/threonine phosphatase involved in the control of numerous cellular processes. Has a catalytic subunit of 36 kDa, a subunit of 55 kDa, and a regulatory subunit of 60 kDa. Reported to associate with microtubules and to bind to purified tubulin polymerized by TAXOL^® Drug.

Legal Information

Taxol is a registered trademark of Bristol-Myers Squibb Co.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hiraga, A. and Tamura, S. 2000. Biochem. J.346, 433.Ferrigno, P., et al. 1993. Mol. Biol. Cell. 4, 669.Guo, H. and Damuni, Z. 1993. Proc. Natl. Acad. Sci. USA90, 2500. Hendrix, P., et al. 1993. J. Biol. Chem.268, 15267.Amick, G.D., et al. 1992. Biochem. J. 287, 1019.Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.Damuni, Z., et al. 1989. J. Biol. Chem.264, 6412.1

Packaging

1, 5 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 14 mM β-ME, 1 mM benzamidine, 1 mM EDTA, 100 µM PMSF, 50% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 nmol of [³²P]P_i from ³²P-labeled phosphorylase per min at 30°C, pH 7.0.

Warning

Toxicity: Standard Handling (A)