General description

Note: 1 mU = 1 milliunit.

Recombinant, human pro-MT2-MMP expressed in E. coli as a periplasmic protein and activated to yield the active catalytic domain (amino acids 91-267). The catalytic domain is comprised of amino acid residues Tyr⁹¹ to Gly²⁶⁷ of mature human MT2-MMP. MT2-MMP is used to study the activation of MMP-2 and the degradation of extracellular matrix proteins.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

D'Ortho, M.-P., et al. 1997. Eur. J. Biochem.250, 751.Kolkenbrock, H., et al. 1997. Biol. Chem.378, 71.Knight, C.G., et al. 1992. FEBS Lett. 296, 263.

Packaging

10 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)