Analysis Note

Positive Control3T3L1 cells treated with LIF

Application

Immunoblotting (1:1000)

General description

Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.

Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr^1022/1023.

This PhosphoDetect™ Anti-JAK1 (pTyr^1022/1023) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK1 (pTyr^1022/1023).

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)

Legal Information

Manufactured by Biosource International.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

A ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.

Liu, K.D., et al. 1997. Curr. Biol.7, 817.Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.

Packaging

10 tests in Plastic ampoule

Physical form

In Dulbecco′s PBS (without Ca²⁺ and Mg²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.

Reconstitution

Following intitial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)