Anti-Hsp90 Mouse mAb (AC88)

Analysis Note

Positive ControlHeat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells

Application

Immunoblotting (1 µg/ml, colorimetric)r>Immunoprecipitation (see comments)r>Immunocytochemistry (see comments)

General description

Recognizes the ~90 kDa Hsp90 protein.

Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Immunogen

Hsp90 isolated from water mold, Achyla ambisexualis

Achyla ambisexualis

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Harry, J.L. et al. 1990. Development190, 305.Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.Riehl, R.M., et al. 1985. Biochemistry 24, 6586.Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)