Application

Immunoblotting (1:1000)

General description

Recognizes G_iα-1 and G_iα-2 subunits. Does not cross-react with G₃α-3 or G_oα subunits.

Protein A purified rabbit polyclonal antibody. Recognizes G_iα-1 and G_iα-2 subunit proteins.

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.

Immunogen

a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian G_iα-1 and G_iα-2, conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with G_iα-3 and G_oα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_iα-1, G_iα-2, G_iα-3, and G_oα. Variables associated with assay conditions will dictate the proper working dilution.

Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Packaging

50 µL in Glass bottle

Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)