General description

Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase preferentially expressed in macrophages and microglia. Exhibits high specific activity.

Note: 1 mU = 1 milliunit.

Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.Kirschke, H., et al. 1989. Biochem. J.264, 467.

Packaging

25 µg in Plastic ampoule

Physical form

In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.

Warning

Toxicity: Harmful (C)