General description

Recombinant, human caspase-8 fused at the N- and C- termini to a His Tag™ sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.

M.W. 11,000 and 18,000 (heterodimer).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Boldin, M.P., et al. 1996. Cell 85, 803.Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.Muzio, M., et al. 1996. Cell 85, 817.

Packaging

3000 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°).

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (catNO=368057">Cat. No. 368057) per min at 30°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)