4G10 MagBead Conjugate

Analysis Note

ControlNIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Application

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10™, Magnetic Bead Conjugate validated for use in IP.

Research CategorySignaling

Research Sub CategoryGeneral Post-translation Modification

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Immunogen

Phosphotyramine-KLH

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

Physical form

20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.

Protein G-Sepharose™ Chromatography

Format: Purified

Quality

100 µg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).

Specificity

This antibody recognizes tyrosine-phosphorylated proteins from all species

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: DO NOT FREEZE

Target description

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.