Analysis Note

ControlHeLa nuclear extract

Application

Use Anti-JMJD1C Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect JMJD1C also known as jumonji domain containing 1C or TR-interacting protein 8.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected JMJD1C in HeLa cells.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryHistone Modifying Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

JMJD1C (jumonji domain-containing protein 1C) is also known as TRIP8 and mutations in this histone demethylase have been linked to certain forms of autism. JMJD1C is also thought to work in reactivating genes in embryonic stem cells, pancreatic islet and gastric cancer. JMJD1C has also been implicated as a gene expression regulator. JMJD1C with WHISTLE have recently been shown to also play a role in mouse testis development. JMJD1C has been identified as a nuclear-receptor coactivator and is a candidate gene for autism.

Immunogen

KLH-conjugated linear peptide corresponding to human JMJD1C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JMJD1C on 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~284 kDa observed