Analysis Note

ControlLysates from transformed E. coli.

Application

Anti-Calmodulin Binding Protein Epitope Tag Antibody detects level of Calmodulin Binding Protein Epitope Tag & has been published & validated for use in WB.

General description

Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.

Immunogen

KLH-conjugated, synthetic peptide (MKRRWKKNFIAVSAANRFKKISSSGAL) corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors. The immunizing sequence is identical in rabbit, mouse, and rat.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4.

Quality

Routinely evaluated by Western Blot in lysates from transformed E. coli.

Western Blot Analysis:
A 1:2000-1:10000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

Specificity

Recognizes recombinant proteins containing the calmodulin binding protein epitope tag.

Target description

The molecular weight is related to the tagged protein.