Analysis Note

ControlSodium butyrate treated HeLa cells

Application

Use Anti-acetyl-Histone H4 (Lys8) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, IP, WB, DB, ChIP-seq to detect acetyl-Histone H4 (Lys8) also known as H4K8Ac, Histone H4 (acetyl K8).

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. r> r>The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Peptide corresponding to amino acids 3-13 of yeast Histone H4 (RGKGG_AcKGLGKG-C) in which lysine 8 is acetylated.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells

Target description

10 kDa