Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

Anti-iPLA2 Antibody is an antibody against iPLA2 for use inWestern Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected iPLA2 in human tonsil tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phospholipase A2 (PLA2) enzymes constitute the largest class of phospholipases, members of which catalyze the hydrolysis of the phospholipid sn-2 ester bond, generating a free fatty acid and a lysophospholipid. PLA2 enzymes are divided into 14 groups (I-XIV), including 20 subgroups (e.g. IA, IB, VIA-1, VIA-2, VIB, etc.), based on sequence homology. Historically, PLA2 enzymes have also been classified based on their biological characteristics into three types, secretory (sPLA2), cytosolic Ca2+-dependent (cPLA2), and cytosolic Ca2+-independent (iPLA2), when sequence data were not available. (PMID 12401193 & 12694560). Cat. No. 07-169-I, Anti-iPLA2, is a purified rabbit polyclonal raised against immunogen sequence present in the group VI SH-iPLA2 & LH-iPLA2 (85/88 kDa calcium-independent phospholipase A2) isoforms, but not found in the 53.2 kDa Ankyrin-iPLA2-1 and 46.5 kDa Ankyrin-iPLA2-2 splice variants encoded by the same PLA2G6 (PLPLA9) gene (UniProt O60733). Immunogen sequence is 100% conserved in murine (NP_058611.1) and rodent (AAC53136.1) species.

Immunogen

Epitope: near C-terminal

KLH-conjugated linear peptide corresponding to human iPLA2 near the C-terminal.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected iPLA2 in 10 µg of human testis tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed (Ramanadham, S., et al. (2003). Biochemistry. 42(47):13929-13940). This antibody is expected to cross-react to isoforms LH-iPLA2 and SH-iPLA2 only.