Analysis Note

ControlJurkat Cell Lysate

Application

Immunohistochemisty (paraffin): GLUT-1 representative staining pattern/morphology on colorectal carcinoma. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select™ Detection with HRP-DAB. GLUT-1 immunoreactivity in erythrocytes.
Note: can be use as internal control for IHC run.

Immunocytochemistry: Recommended

ELISA: Recommended

Western Blot: A previous lot of this antibody was tested on multiple lysates using western blot analysis. See datasheet.

Research CategorySignaling

Anti-GLUT-1 Antibody, C-terminus is an antibody against C terminus of GLUT-1 that is validated for the detection of GLUT-1 in Western Blotting (WB), Immunohistochemistry - Paraffin (IHC-P), Immunocytochemistry (ICC) and ELISA.

Research Sub CategoryInsulin/Energy Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose.

Immunogen

Epitope: C-Terminus

Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV).

Linkage

Replaces: AB1340

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal IgG in buffer containing 0.02 M Phosphate Buffer, 0.25 M NaCl, with 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HUVEC.

Western Blotting Analysis: 1 µg/mL of this antibody detected GLUT-1, CT in HUVEC.

Specificity

This rabbit polyclonal antibody detects GLUT-1 in human and mouse cells. It targets an epitope within 12 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

This antibody detects a major band ~54 kDa in Western blot analysis.